Figure 3.

APS-IgG do not increase the phosphorylation of p38 MAPK, NFκB p65 or ERK. HTR-8 cells were treated with 100 μg/mL pooled IgG from VT+/PM− with 78.2GPLU and 44.4SU binding activity, VT−/PM+ with 83.4GPLU and 71.5SU binding activity and HCs with 0GPLU and SU binding as well as the positive controls TNF-α (10 ng/mL). Graphs show relative expression at 15 min of p38 MAPK (a), NFκB p65 (b) and ERK (c). The mean ± SEM of quantitative analysis from three independent experiments is shown. No statistically significant differences were found by one-way anova.