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. 2015 Feb 15;118(4):940–953. doi: 10.1111/jam.12759

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© 2015 The Authors published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Rarefaction curves (a), nonmetric multidimentional scaling (b) of eight eukaryotic communities at a 3% cut-off and community composition at the kingdom level (c). All the datasets were normalized to have 3108 reads. The samples before pH adjustment are shown as ‘’ and after pH adjustment are represented by ‘’ (b). (c) All the reads which obtained using the primer set euk7F-euk570R were aligned to the SILVA database, and are shown as relative percent abundance. The eukaryotic groups are: Fungi (♦), Amoebozoa (▴) and Unclassified (▪). () Md13E; () Md23E () Md40E () Md82E; ()Md103E; () Md110E; () Md172E and () Md201E.

Inline graphic — Rarefaction curves (a), nonmetric multidimentional scaling (b) of eight eukaryotic communities at a 3% cut-off and community composition at the kingdom level (c). All the datasets were normalized to have 3108 reads. The samples before pH adjustment are shown as ‘’ and after pH adjustment are represented by ‘’ (b). (c) All the reads which obtained using the primer set euk7F-euk570R were aligned to the SILVA database, and are shown as relative percent abundance. The eukaryotic groups are: Fungi (♦), Amoebozoa (▴) and Unclassified (▪). () Md13E; () Md23E () Md40E () Md82E; ()Md103E; () Md110E; () Md172E and () Md201E.