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. Author manuscript; available in PMC: 2015 Apr 24.
Published in final edited form as: Dev Neurobiol. 2008 Nov;68(13):1441–1453. doi: 10.1002/dneu.20670

Fig. 4. NAV2 protein is localized in the cell body and along the length of the growing neurites in RA-treated SH-SY5Y cells.

Fig. 4

(A) Epifluorescence images of SH-SY5Y cells exposed to vehicle or atRA for 72 h, and stained with affinity-purified NAV2 antibody or rabbit IgG. (B) Epifluorescent images of atRA treated SH-SY5Y cells (72 h) stained with NAV2 affinity-purified antibody (green in merged panel) along with anti α-tubulin (red in merged panel). (C) Epifluorescent images of atRA-treated (72 h) SH-SY5Y cells stained with affinity purified NAV2 antibody (green in merged panel) and an antibody to phosphorylated medium chain neurofilament (RMO270, red in merged panel). A region where the neurite is emerging from the cell is shown below each panel in which the light intensity levels are optimized to show staining in the neurite. (D) Confocal analysis of NAV2 (green) and neurofilament (red) protein show punctate NAV2 protein distribution in cell body and along the length of the neurite co-localizing with neurofilament protein which appears as smooth fibers. The merged image shows colocalization of NAV2 immunostaining with neurofilament in SH-SY5Y cells (yellow).