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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Immunol. 2014 Dec 22;194(3):1112–1121. doi: 10.4049/jimmunol.1401958

FIGURE 1. Pa induces apoptosis of macrophages in a dose dependent manner.

FIGURE 1

MH-S cells were transfected with control (Ctrl) siRNA or Atg7 siRNA for 24 h. Murine primary macrophages were isolated using bronchoalveolar lavage (BAL) procedures. Cells were infected with different amounts of PAO1 for 2 h. (A) Cell viability index was measured by MTT assay. (B) Mitochondrial potential was assessed by the JC-1 fluorescence assay (0.1 μg/ml). The fluorescence was quantified at 532 nm with a fluorimeter. (C) Immunoblotting analysis of PARP, caspase3, Bcl-2, Bax and cytochrome C using lysates of MH-S cells after infected with various amounts of Pa. β-actin was used as the loading control throughout the manuscript. (D) TUNEL assay was performed to measure the ratio of apoptotic cells. TUNEL positive cells were counted from at least 100 random fields. Scale bars=100 μm. Data are representative as means±SD of three independent experiments (*, p<0.05; **, p<0.01). RFU, relative fluorescence units.