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. 2015 Apr 24;10(4):e0125104. doi: 10.1371/journal.pone.0125104

Fig 3. Expression of type II and III phosphate transporter isoforms during osteoclast differentiation of RAW 264.7 cells.

Fig 3

RAW 264.7 cells were cultured with or without RANKL for 6 days. A-E) NaPi transcript expression was assessed by real-time PCR analysis. F, G) Calcitonin receptor (CTR) and cathepsin K (CTSK) were used as markers of osteoclast differentiation. No signal was detected for NaPi-IIa, NaPi-IIb and NaPi-IIc at any time point irrespective of the presence or absence of RANKL (not shown). Positive control PCRs in A-E were done with kidney cDNA for NaPi-IIa, NaPi-IIc, Pit-1 and Pit-2 and lung cDNA for NaPi-IIb. The amount of mRNA relative to GAPDH was calculated using the ΔCt method. Values are shown as means ±SD; n = 3 independent experiments/group. * p <0.05; NS, not significant.