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Representative ITC data of BhCBM46, BhCel5B_E296A, and variants binding to soluble ligands. Titrations were conducted in 50 mm Na-HEPES buffer, pH 7.5, containing 200 mm NaCl at 25 °C. Top, ligand barley β-glucan in the syringe was titrated into cell contained protein (50 μm). Bottom, ligand xyloglucan in the syringe was titrated into cell contained protein (50 μm).
