FIGURE 1.

Inhibition of PP2A by okadaic acid treatment increases AMP kinase phosphorylation and activity. A, total cell extracts from A7r5 cells were obtained and treated with the concentrations of OA indicated. PPP2A activity was determined using an ELISA Ppp2ca phosphatase assay kit from R&D Systems. B, A7r5 cells were treated with 10 nm okadaic acid, and cell extracts were obtained. The levels of pAMPKα, AMP kinase, and Acc1 (pAcc1) phosphorylation were determined using specific antibodies generated to recognize the phosphorylation status of AMP kinase α Thr-172, total AMP kinase, and Acc1 Ser-79. Error bars represent S.E.