FIGURE 10.
PPPPpp2r2d level increases in the aorta of mice fed a high fat diet. C57BL/6 mice were fed a high fat diet as described under “Experimental Procedures.” A, the weight of all mice was determined at the indicated times (n = 8). B, blood cholesterol levels of all mice were determined at the indicated times using a cholesterol level assay kit from Stanbio. C, blood triglyceride levels of individual mice were determined at the indicated times using a triglyceride level assay kit from Stanbio. D, blood total LDL levels of individual mice were determined at the indicated times using an LDL level assay kit from Stanbio. E, F, and G, protein expression levels of various proteins were determined in aortas at the indicated times using Western analysis in high fat-fed mice. The antibodies used were specific for each PP2A subunit, AMP kinase, phosphorylated AMP kinase, and phosphorylated Acc1 (pAcc1), and they were tested for any cross-reactivity using siRNA knockdown experiments. Actin was used as a loading control. The data from three individual mice are shown. Closed circles, WT, normal chow; open circles, WT, high fat diet. 7%, 7% fat diet; 21%, 21% fat diet. Error bars represent S.E.