FIGURE 4.
Loss of PP2APpp2r2d, but not PP2APpp2r5e, results in increased AMP kinase Thr-172 phosphorylation and activity. A, A7r5 cells were treated with control siRNA (Csi) or siRNA directed against PPP2R2D or PPP2R5E, and PP2A activity was determined using the PP2A activity assay kit from R&D Systems. B, A7r5 cells were treated with control siRNA (Csi) or siRNA directed against PPP2CA or PPP2CB, and protein level and phosphorylation status were determined by Western analysis (WB). C, A7r5 cells were treated with control siRNA (Csi) or siRNA (si) directed against PPP2R1A or PPP2R1B, and protein level and phosphorylation status were determined by Western analysis. D, A7r5 cells were treated with control siRNA (Csi) or siRNA directed against PPP2R2D or PPP2R5E, and protein level and phosphorylation status were determined by Western analysis. Actin was used as a loading control. Error bars represent S.E.