FIGURE 8.
PP2APpp2r2d forms a complex with AMP kinase α and Srebp1 and regulates Srebp1-dependent gene expression. A, A7r5 cells were treated with control (Csi) or PPP2R2D siRNA (PPP2R2Dsi). The levels of Ppp2r2d and mature Srebp1 were determined using Western analysis. Actin was used as a loading control. B, A7r5 cells were grown in the absence (Csi) or presence of PPP2R2D siRNA (PPP2R2Dsi). The level of Srebp1-dependent PPARγ mRNA expression was determined by quantitative RT-PCR. C, 10% of the total protein used for co-immunoprecipitation experiments. Actin was used as a loading control. D, cell extracts were incubated with AMP kinase or Srebp1 antibodies (IP), and associated proteins were isolated using Protein A-Sepharose beads. Co-immunoprecipitated proteins were resolved by SDS-PAGE and detected using Western analysis (WB) using the indicated antibodies.