FIGURE 3.

SALL4 affects ATRA target gene expression. A, in HEK293 cells, the RARβ promoter activity under different treatments was measured 24 h after plasmid transfection and Dox administration (shown as micrograms per milliliter). Relative luciferase units (RLU) are expressed as the mean ± S.E. (n = 3). Ctrl, control. B, the effects of SALL4 knockdown on the expression of ATRA-target genes. HL60 cells were treated with the indicated drugs for 30 h. The relative mRNA levels of the indicated genes were determined by qRT-PCR and quantified on the basis of three independent experiments. Error bars denote S.D. *, p < 0.05; **, p < 0.01 versus ATRA only.