FIGURE 7.

SALL4 differentially interferes with the ATRA-RA pathways in APL and non-APL AML cells. A, comparison of ALL4 protein expression in HL60 and NB4 cells after ATRA treatment at different time points or with different dosages by Western blotting. β-Actin was used as an internal loading control. B, co-IP experiments to detect potential SALL4 and PML-RARα interaction in NB4 cells. HL60 and NB4 cell nuclear extracts (200 μg) were immunoprecipitated with IP-grade SALL4 antibody and Western-blotted with anti-PML/RARα and anti-RARα antibodies, respectively. The experiments were repeated at least twice, and similar results were obtained. IB, immunoblot. C, a tentative model for the molecular interaction of SALL4 with RARα/RXR and PML-RARα in APL and non-APL AMLs. See text for details.