FIGURE 5.

SDS-PAGE analysis of tāpirin binding to various plant cell wall components and plant biomass. Tāpirins tested include Csac_1073 (class 1) (A), Calkro_0844 (class 1) (B), and Calkro_0845 (class 2) (C), and thermolysin-digested Calkro_0844 (Calkro_0844_C) (D). Abbreviations for plant biomass substrates are as follows: aSWG, dilute acid-pretreated switchgrass; aPTD, dilute acid-pretreated P. deltoides × P. trichocarpa; PTD, P. deltoides × P. trichocarpa. B, bound protein liberated from the substrate after boiling in 1× Laemmli buffer; U, free protein. 40 μg of protein was used in all conditions tested; image is representative of three replicates.