FIGURE 6.

Binding isotherm of selected tāpirin to filter paper and Avicel. MES (50 mm), pH 7.2, NaCl (3.9 mm) as used as the binding buffer. Equilibrium association constant (K_a, μm⁻¹) and binding capacity (B_max, μmol g⁻¹) for each protein and substrate combination are indicated in the inset table. Binding isotherms are represented by the following: solid circles for Calkro_0844 and Avicel; empty circles for Calkro_0844 and filter paper; solid triangles for Calkro_0845 and Avicel; empty triangles for Calkro_0845 and filter paper.