TABLE 1.
Primers used in this study
Underlined sequences are either vector-specific or restriction enzyme sites.
| Primer name | Sequence | Cloning vector |
|---|---|---|
| Csac_1073 F | GACGACGACAAGATGTCTGCTGTGTTGGCATCACTG | pET46 Ek/LIC |
| Csac_1073 R | GAGGAGAAGCCCGGTTACTTTATAACAATGTTTCGTC | pET46 Ek/LIC |
| Calkro_0844 F | GACGACGACAAGATGTCGGCTGTGTTAGCATCGCTG | pET46 Ek/LIC |
| Calkro_0844 R | GAGGAGAAGCCCGGTTACCTTGTAACCATGTTTCGTC | pET46 Ek/LIC |
| Calkro_0845F | GACGACGACAAGATGTCGGCTGTGTTAGCATCGCTG | pET46 Ek/LIC |
| Calkro_0845R | GAGGAGAAGCCCGGTTATTTAACAACAATACTTCTTC | pET46 Ek/LIC |
| Calkro_0844 F GA | GGTGGTTCTGCTAGCGCATCGCTGAACCAGAGCACATCTATA | pCTCON |
| Calkro_0844 R GA | GCTTTTGTTCGGATCCCCTTGTAACCATGTTTCGTCTTAA | pCTCON |
| pCTCON F-BamHI | GACGAAACATGGTTACAAGGGGATCCGAACAAAAGCTTATTTCTGAAGAG | pCTCON |
| pCTCON R-NheI | GCTCTGGTTCAGCGATGCGCTAGCAGAACCACCACCACCAGA | pCTCON |
| YSD-Calkro_0845-F | GTCAGCTAGCATGTCGGCTGTGTTAGCATCGCTGAA | pCTCON |
| YSD-Calkro_0845-R | GTCACTCGAGCTATTATTTAACAACAATACTTCTTCTTAC | pCTCON |