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. 2015 Mar 6;290(17):10657–10666. doi: 10.1074/jbc.M114.626911

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Time course of [³⁵S]gpαf transport and Sec22(L213C)-Sec22HA(L213C) mixed dimer formation. Transport reactions were assembled with Sec22(L213C) vesicles containing [³⁵S]gpαf and Sec22HA(L213C) acceptor membranes in the presence of fusion factors and an ATP regeneration system. The reactions were incubated at 23 °C for various times and then processed to determine the amount of Golgi-modified [³⁵S]gpαf (closed circles) or Sec22-Sec22HA mixed dimer (closed squares). The amounts of α1,6-mannose precipitable [³⁵S]gpαf and of mixed Sec22 dimer from the immunoblot (inset) were plotted as a percentage of maximum levels over time. Sly1 immunoblot serves as a loading control.