FIGURE 6.

Residues identified as binding sites around basic patch. Error bars, S.D. from a triplicate set of experiments. a, residue level extent of oxidation for peptide 70–81 of Robo1 (black bars) and Robo1-heparin (gray bars). b, residue level extent of oxidation for peptide 151–169 of Robo1 (black bars) and Robo1-heparin (gray bars). c, structure shows the protected residues Ser⁸⁰-Lys⁸¹ and Ala¹⁶⁶-Ile¹⁶⁷-Leu¹⁶⁸-Arg¹⁶⁹ (red) are at a basic patch with basic residues in magenta unidentified by HRPF. Asterisks indicate the residue identified as interacting with heparin dp8 previously (21).