FIGURE 7.

Inhibitor of PI3K-induced AKT Ser⁴⁷³ phosphorylation increases GRB10 expression. A, Ser(P)⁴⁷³-AKT was reduced in the HepG2 cells following exposure to LY294002 (50 μm × 24 h), an inhibitor of PI3K-mediated phosphorylation of AKT (*** for p < 0.001; n = 3). B, decline in Ser⁴⁰¹ GATA2 phosphorylation was also evident following exposure to LY294002 (*** for p < 0.001; n = 3). This is consistent with the observed positive correlation between Ser(P)⁴⁰¹-GATA2 and Ser(P)⁴⁷³-AKT. C, GRB10 mRNA expression, detected by QPCR, increased significantly following inhibition of AKT phosphorylation by LY294002 (*** for p < 0.001; n = 6). D, GRB10 protein, determined by ELISA, increased significantly following treatment with LY294002 (*** for p < 0.001, n = 3). CGN, carrageenan; LY, LY294002.