TABLE 1.
Met oxidation was quantified by LC-MS as described under “Experimental Procedures”
The percentage of oxidation of single Met residues is reported as the mean and S.E. n is the number of independent experiments as defined under “Experimental Procedures.” N/A, non-applicable for the specific protein variant.
| Sample name | Oxidation System | H2O2:apoA-I (mol:mol) | Met(O)86 (n) | Met(O)112 (n) | Met(O)148 (n) |
|---|---|---|---|---|---|
| Mean (%) ± S.E. | Mean (%) ± S.E. | Mean (%) ± S.E. | |||
| Intact plasma apoA-I | 1.3 ± 0.5 (n = 3) | 2.3 ± 1.0 (n = 3) | 2.7 ± 0.2 (n = 3) | ||
| Ctrl plasma apoA-I | None | 0:1 | 1.6 ± 0.8 (n = 6) | 2.7 ± 0.5 (n = 7) | 1.6 ± 0.3 (n = 7) |
| MPO-2:1-H2O2-apoA-I | MPO-H2O2-Cl− | 2:1 | 41.6 ± 5.9 (n = 5) | 76.0 ± 4.1 (n = 5) | 57.8 ± 4.6 (n = 5) |
| MPO-3:1-H2O2-apoA-I | MPO-H2O2-Cl− | 3:1 | 59.9 ± 5.0 (n = 4) | 96.6 ± 1.4 (n = 4) | 86.6 ± 4.0 (n = 4) |
| H2O2-low-apoA-I | H2O2 | 750:1 | 67.5 ± 5.6 (n = 4) | 99.8 ± 0.2 (n = 4) | 94.5 ± 3.1 (n = 4) |
| H2O2-high-apoA-I | H2O2 | 1000:1 | 93.0 ± 2.5 (n = 4) | 100.0 ± 0.0 (n = 4) | 98.1 ± 1.3 (n = 4) |
| Ctrl rapoA-I | 0.4 ± 0.0 (n = 3) | 2.5 ± 0.4 (n = 6) | 4.2 ± 1.0 (n = 6) | ||
| H2O2-rapoA-I | H2O2 | 750:1 | 70.7 ± 5.2 (n = 5) | 98.9 ± 0.5 (n = 5) | 86.3 ± 2.2 (n = 5) |
| H2O2-M86L-rapoA-I | H2O2 | 750:1 | N/A | 99.9 ± 0.1 (n = 3) | 94.5 ± 2.2 (n = 3) |
| H2O2-M112L-rapoA-I | H2O2 | 750:1 | 70.9 ± 3.3 (n = 3) | N/A | 86.9 ± 1.9 (n = 3) |
| H2O2-M148L-rapoA-I | H2O2 | 750:1 | 37.9 ± 2.8 (n = 6) | 99.9 ± 0.1 (n = 3) | N/A |