TABLE 2.
Specificity constant kcat/Km for Mtb ClpP1P2 and E. coli, B. subtilis, and S. aureus ClpPs against tripeptide-amc substrates
Nle, norleucine.
| Substrate X3X2X1-amc |
kcat/Km |
|||
|---|---|---|---|---|
| M. tuberculosis | S. aureus | B. subtilis | E. coli | |
| m−1 s−1 | ||||
| Chymotryptic | ||||
| Ac-PKM | 1327 | 331 | 176 | 2632 |
| Ac-PKNle | 411 | 69 | 239 | 2464 |
| Ac-KM | 144 | 56 | 36 | 630 |
| Ac-PWM | 1155 | 462 | 520 | 4745 |
| Ac-WM | 612 | 184 | 268 | 4095 |
| Ac-ARM | 410 | 236 | 526 | 1553 |
| Ac-HKM | 169 | 128 | 6 | 695 |
| Ac-PKL | 199 | 270 | 234 | 2021 |
| Ac-PQL | 63 | 183 | 247 | 2886 |
| Ac-PYL | 32 | 265 | 598 | 3302 |
| Ac-PAL | 42 | 127 | 282 | 1804 |
| Ac-PKF | 320 | 185 | 132 | 634 |
| Ac-PAF | 68 | 62 | 97 | 605 |
| Ac-PKA | 40 | 35 | 76 | 812 |
| Z-LLL | 0 | 14 | 0 | 46 |
| Z-LL | 0 | 8 | 2 | 58 |
| Suc-LY | 1 | 7 | 50 | 721 |
| Suc-LLVY | 2 | 2 | 1 | 26 |
| Tryptic | ||||
| Ac-WEK | 2 | 0 | 0 | 3 |
| Ac-FAK | 1 | 0 | 0 | 0 |
| YMK | 2 | 0 | 2 | 1 |
| Z-LLR | 5 | 0 | 1 | 1 |
| Z-FVR | 4 | 1 | 0 | 1 |
| Acidic | ||||
| Z-nLPnLD | 14 | 3 | 10 | 31 |
| Z-APnLD | 0 | 0 | 0 | 0 |
| Ac-PnLD | 0 | 4 | 0 | 0 |
| Ac-LWD | 2 | 1 | 0 | 14 |
| Ac-WID | 0 | 2 | 0 | 0 |