FIGURE 3.

Chemical ablation of iPLA₂β promotes and iPLA₂β overexpression suppresses selection of downstream 5′SS in human Bcl-x minigene. A, representative immunoblot showing iPLA₂β protein levels in INS-1 cells transfected with empty vector (V) or iPLA₂β cDNA (OE). B, empty vector and OE INS-1 cells were transfected with plasmid expressing a functional human Bcl-x minigene. Cells were cultured for 13–16 h in the presence of DMSO (c) or (S)-BEL (10 μm). RNA was harvested, and RT-PCR performed to amplify minigene splice variants. Shown are a representative experiment (left panel) and the quantification of four independent experiments (right panel). (*, significantly different from c-treated INS-1 vector cells, p < 0.05; #, significantly different from c-treated INS-1 cells, p < 0.05.) Each quantification is presented as mean ± S.E.