FIGURE 5.

iPLA₂β promotes selection of the upstream alternative 5′SS in endogenous islet Bcl-x. Islets were harvested from wild-type (WT), RIP-iPLA₂β-Tg (Tg), and iPLA₂β^−/− (KO) mice. A, immunohistochemistry analysis of iPLA₂β (red) and insulin (green). Islets were counterstained with DAPI (blue) to mark the nuclei of individual cells. Merged images are presented, where arrowheads indicate co-expression of iPLA₂β and insulin. B, islets were isolated and then cultured in the presence of DMSO (−) or 2 μm thapsigargin (+). RNA was harvested and used for RT-PCR to amplify Bcl-x splice variants. Two mice were studied in each group, and results from both mice are shown. The average ratio of Bcl-x(L)/Bcl-x(S) is shown for each treatment group.