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Generation of CCR5/CCR2b receptor chimeras. A, schematic structure of chimeras 2252, 2255, and 2b199–207R5; the CCR5 component of the chimera is shown in black. B, expression of the chimeric receptor 2252 in HEK293F cells by flow cytometry. The chimera is recognized by antibodies specific for ECL2 of CCR5 (2D7) and CCR2b but not by the antibody to the N terminus of CCR5 (3A9).
