FIGURE 4.

DDIT3 enhances TNFRSF10A transcription via DDIT3·p-JUN complex. A, H1792 cells were treated with DMSO, TG (1 μm), or Tm (1 μm) for 12 h, and cell lysates were immunoprecipitated (IP) using the anti-DDIT3 antibody. The isotype IgG₁ antibody served as control. The indicated proteins were detected using Western blot analysis. p-JUN, phospho-Jun; p-FOS, phospho-FOS. B, H1792 cells were treated with DMSO, TG (1 μm), or Tm (1 μm) for 12 h, and then the cell lysates were prepared and ChIP assays were performed using the anti-DDIT3 and anti-phospho-JUN antibodies and using the isotype IgG antibody as negative control (data not show). The input and immunoprecipitated samples were used as template for PCR amplification of fragments containing the AP-1 binding site, and GAPDH was used as a loading control.