Conditioned medium from C26 cancer cells activates p-Stat3 in C2C12 myotubes, a model of skeletal muscle.
A, representative Western blots for p-Stat3 and Stat3 exposed to the conditioned medium of C26 for different times. B, representative Western blots for p-Stat3 or Stat3 with or without C188-9 in conditioned medium for 15 min. C, the average sizes of C2C12 myotubes following a 72-h incubation in C26-conditioned medium with or without C188-9. Error bars indicate means ± S.E. D, representative Western blots of caspase-3 and cleaved caspase-3 of C2C12 incubated in C26-conditioned medium. E, representative Western blots of pro-caspase-3 in C2C12 myotubes treated with C26-conditioned medium with or without C188-9. F, representative Western blots of C/EBPδ and myostatin in C2C12 myotubes treated with C26-conditioned medium with or without C188-9. CTRL, control.