FIGURE 3.
Muscle-specific Stat3 KO in mice suppresses LLC-induced loss of muscle mass. Mice with muscle-specific Stat3 KO (KO) or control mice (Stat3flox/flox (F/F)) were used; 10 mice in each group were injected with LLC cells. A–G, after 18 days, we assessed: body weight (A); weights of gastrocnemius (Gast.) muscles (B); differences in cross-sections of tibialis anterior muscles that were immunostained with anti-laminin (C); the average sizes of myofibers (D); weights of tumors (E); muscle grip (mean values ± S.E.) (F); and representative Western blots of Stat3, C/EBPδ, and myostatin in gastrocnemius muscles of Stat3 KO mice versus control, floxed Stat3 (F/F mice) that had been treated with and without LLC tumors (G). CTRL, control. Densities of these Western blots were corrected for GAPDH and quantified (bar graphs).