FIGURE 4.

Elimination of C/EBPδ in mice suppresses LLC-induced cachexia. A–F, C/EBPδ KO and control mice were injected with LLC cells. Eighteen days later, we measured: body weight (A); weights of gastrocnemius (Gast.) (B); cross-sections of tibialis anterior muscles following immunostaining with anti-laminin (C); average myofiber sizes (D); rates of muscle protein degradation (E); and muscle grip strength (F). CTRL, control. G, representative Western blots of myostatin in muscles of C/EBPδ KO or control mice inoculated with or without LLC (upper panel) are shown. The -fold changes in myostatin expression when compared with results in control mice are shown (lower panel, quantification of myostatin levels corrected for GAPDH). H, representative Western blots of 14-kDa actin fragment in muscles of wild type or C/EBPδ KO mice bearing tumors. The -fold changes in 14 kDa to total actin are shown (lower panel,* p < 0.05 versus non-tumor mice). Results are reported as mean ± S.E. NS, not significant.