Fig. 2.

Hepatic contents of intermediates of the cholesterol synthetic pathway in control and L-SSKO mice. The liver tissue lysates from male mice at the age of 12 weeks (n = 5 in each group) were analyzed. Mevalonate content (A) was determined by the HMGCR enzymatic cycling assay. Squalene content (C) was determined by HPLC. Cholesterol content (H) was determined enzymatically. Other intermediates (B, D–G) were determined by LC/MS/MS method. Each value represents the mean ± SD. * P < 0.05 and ** P < 0.001 by Student’s t-test.