Fig. 4.

Plasma lipoprotein metabolism in control and L-SSKO mice. Total cholesterol (A) and triglyceride (B) contents in lipoproteins were determined by HPLC analysis. The chromatographic patterns of mean values from fSS (gray line, n = 5) and L-SSKO (black line, n = 5) male mice at 12 weeks of age. C: Immunoblot analysis of plasma apoB for fSS and L-SSKO male mice at the age of 12 weeks. Two microliters of plasma taken from mice in the regular chow-fed state was subjected to SDS-PAGE. Each lane represents an individual mouse (n = 3, in each group). D: Hepatic VLDL production rate after injection of Triton WR1339 in fSS (n = 4) and L-SSKO (n = 6) male mice at the age of 12 weeks. This rate is expressed as the increase in plasma triglyceride levels. E: Decay of ¹²⁵I-labeled rabbit β-VLDL from the plasma in fSS, L-SSKO, and LDLR KO male mice at the age of 12 weeks (n = 5 in each groups). Each value represents the mean ± SD. * P < 0.01 by two-way repeated-measures ANOVA in E.