Fig. 5.

Hepatic expression of mRNA and proteins involved in lipid metabolism and hepatic HMGCR activity. A: Total RNA from the liver of male mice (n = 5 in each group) at the age of 12 weeks was subjected to quantitative real-time PCR. Relative mRNA levels were normalized to GAPDH. B: Immunoblot analysis of LDLR, SR-BI, and HMGCR of fSS and L-SSKO male mice at the age of 12 weeks. PDI was served as loading controls. Each lane represents an individual mouse. C: HMGCR activity (the rate of biosynthesis of mevalonate from HMG-CoA) in the liver microsomal fractions of fSS and L-SSKO male mice at 12 weeks of age (n = 4 in each group). Each value represents the mean ± SD. * P < 0.05, ** P < 0.01, and *** P < 0.001 by Student’s t-test. LRP1, LDL receptor-related protein 1; MTTP, microsomal triglyceride transfer protein.