Table 2. Results of qPCR for detection of RLW DNA in blood or tissue of rats involved in Trial I and Trial II.

Trial I n = 5 rats/treatment				Trial II n = 2 rats/treatment
Treatment	Time PI	Number of qPCR replicates/ rat 1	Number of rats positive 2	Time PI	Number of qPCR replicates/ rat 3	Number of rats positive 2
Control	pre-bleed	4	0	pre-bleed	4, 8	0
	4–7 hrs	4	0	45–55 mins	4	0
	21–24 hrs	4	0	2–2:20 hrs	4	0
	day 2	4	0	14–15 hrs	4	0
	day 3	4	0	16–17 hrs	4	0
	week 1	4	0	18–19 hrs	4	0
	week 2	4	0	Week 4	2, 4	0
	wk 6 cardiac	2	0	Week 5	2	0
	wk 6 tail	8	0	Week 6	2, 4	0
Low dose	pre-bleed	4	0	pre-bleed	2	0
	4–7 hrs	4	0	30–35 mins	2	0
	21–24 hrs	4	0	1:30 hrs	2, 6	1
	day 2	4	0	14–15 hrs	2	0
	day 3	4	0	16–17 hrs	2	0
	week 1	4	0	18–19 hrs	2	0
	week 2	4	0	Week 4	2, 4	0
	week 6 cardiac	2	5	Week 5	4	1
	wk 6 tail	12	3	Week 6	2, 4	2
High dose	pre-bleed	4	0	pre-bleed	2	0
	4–7 hrs	4	0	50–60 mins	2, 6	1
	21–24 hrs	4	0	2–2:15 hrs	2	0
	day 2	4	0	14–15 hrs	2	0
	day 3	4	0	16–17 hrs	2	0
	week 1	4	0	18–19 hrs	2	1
	week 2	4	0	Week 4	2	0
	week 6 cardiac	2	5	Week 5	4, 5	2
	wk 6 tail	12	5	Week 6	2	2

High dose infection (HD, 50 larvae/ml gavage), low dose (LD, 10 larvae/ml gavage), control (C, 0 larvae/ml gavage).

¹One extraction per individual except wk 6 tail: tail tissue, which had two extractions per individual for controls, 3 extractions per individual for infected.

²Positive = clear signal in large proportion of replicates

³If the number of replicate qPCR tests differed between the two individuals, they are reported separately.