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Study design for Fib-Gen repair using IVD organ culture model. (a) Bovine caudal IVD samples that retained IVD endplates were evaluated as Intact, Injured (with simulated 4.5 × 4.5 mm box-cut defect) and Repaired (injured with Fib-Gen injection for repair). (b) Hydraulically controlled dynamic compression bioreactor with culture chambers. (c) Simulated daily activity load consisted of 8 h of cyclic compression and 16 h of static resting load.
