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. 2015 Apr 24;11(4):e1005197. doi: 10.1371/journal.pgen.1005197

Fig 1. Comparisons of the effects of deleting Hel2 and Ltn1.

Fig 1

(A) Western blot analysis for Rluc-HIS3 reporters from the wild-type (WT), hel2 ∆, and ltn1 ∆ strains (BY4727, SKY61, S18-E01). A schematic image of reporter fusion genes, Rluc-HIS3, either with or without 12 CGA codon repeats, is illustrated above. The expression of fusion protein was detected using a Rluc antibody. PGK1 was used as a loading control. Arrowhead 1 indicates the Rluc protein alone, arrowhead 2 indicates the Rluc-blank-HIS3 protein, and arrowhead 3 indicates the Rluc-CGA x12-HIS3 protein. (B) Dual luciferase assay for Rluc-CGA x12-luc2 reporter in the wild-type (WT), hel2 ∆, and ltn1 ∆ strains (HRKW-2, SKY113, HRKW-6). A schematic image of reporter fusion genes, Rluc-luc2, either with or without 12 CGA codon repeats, is illustrated above. Bars indicate luc2/Rluc ratios. Percentages were standardized using the results of a dual luciferase assay for Rluc-blank-luc2 in the wild-type strain (HRKW-1). The inset indicates the results with the ltn1 ∆ strain with an appropriately adjusted range. Average luc2/Rluc ratios and standard deviations were determined from three independent measurements.