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. 2015 Apr 24;11(4):e1005197. doi: 10.1371/journal.pgen.1005197

Fig 3. Comparison of the effects of Hel2 deletion and K63R expression on protein and mRNA levels in stalled translation.

Fig 3

(A) Western blot analysis for the effects of K63R ubiquitin on Rluc-HIS3 reporter. blank indicates the result of the Rluc-blank-HIS3 reporter, and CGAx12 indicates the results of the Rluc-CAAx12-HIS3 reporter. WT indicates the wild-type strain (BY4727) without K63R ubiquitin, hel2∆ indicates the hel2∆ strain (SKY61), and K63R indicates the wid-type strain expressing K63R ubiquitin. Arrowhead 1 indicates the Rluc protein alone, arrowhead 2 indicates the Rluc-blank-HIS3 protein, and arrowhead 3 indicates the Rluc-CGA x12-HIS3 protein. PGK antibody was used as loading control. (B) Northern blot analysis for the effects of K63R ubiquitin on Rluc-HIS3 reporter. blank and CGAx12 as well as WT, hel2∆, and K63R are as in (A). The reporter mRNA was probed by HIS3 complementary DNA fragment. Arrowhead 1 indicates the cleaved HIS3 mRNA and arrowhead 2 indicates the full-length mRNA. SCR1 mRNA was detected as a loading control.