Skip to main content
. 2015 Apr 24;10(4):e0124266. doi: 10.1371/journal.pone.0124266

Fig 6. CDC25A induces the G1-to-S phase transition in HCC cells.

Fig 6

(A,B) Western blotting of CDC25A protein expression in HepG2 cells infected/transfected with lenti-CDC25A, lenti-control, CDC25A-siRNA or negative control-siRNA. (C) Western blot analysis of CDC25A expression in SMMC-7721 cells and SMMC-7721-let-7c stable cells with or without CDC25A* reintroduction. (D,E) Infection/transfection of HepG2 cells with lenti-CDC25A, lenti-control, CDC25A-siRNA or negative control-siRNA was performed to investigate the effects of CDC25A on the HCC cell cycle. Representative images are shown. (F) Cell cycle assays of SMMC-7721 cells or SMMC-7721-let-7c stable cells with or without CDC25A* reintroduction. Restoration of CDC25A significantly induced the G1-to-S phase transition in SMMC-7721 cells. Representative images are shown. *CDC25A was reintroduced without its 3′-UTR to prevent the expression of CDC25A from being inhibited by let-7c.