Table 3. A comparison of the STY2879-LAMP and RT-PCR assays in the human stool samples spiked with two low levels of S. Typhi.
Assay | Bacterial concentration (CFU/g) | Enrichment time (hours) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | ||
LAMP Tt (min) | 2 | - | - | - | - | - | 35.2 | 32.5 | 30.3 | 27.4 | 25.1 | 20.8 |
20 | - | - | - | - | 32.2 | 30.1 | 28.0 | 25.7 | 22.1 | 19.4 | 16.2 | |
RT-PCR Ct (cycles) | 2 | - | - | - | - | - | - | - | - | 35.5 | 31.0 | 27.2 |
20 | - | - | - | - | - | - | - | 36.8 | 31.3 | 27.3 | 26.4 |
“-” means that a positive result with this sample is not available.