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. 2015 Apr 24;10(4):e0124664. doi: 10.1371/journal.pone.0124664

Fig 1. Diagram of preparing recombinant OmpB fragments (rOmpBs).

Fig 1

The full-length sequence of ompB was divided into 5 fragments (named as ompB-1 to -5) according to hydrophilicity, antigenic index, and surface probability (A). Five recombinant OmpB fragments (named as rOmpB-1 to -5) purified from E. coli cell lysate were separated by 10% SDS-PAGE and stained by G-250 Coomassie Brilliant Blue (B) and immunoblotted with sera from mice infected with R. rickettsii (C): lane M, protein molecular mass markers; lanes 1 to 5, rOmpB-1 to -5 (C).