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. 2015 Apr 24;10(4):e0124664. doi: 10.1371/journal.pone.0124664

Fig 2. Evaluation of immunoprotective efficacy of rOmpBs.

Fig 2

C3H/HeN mice were immunized with each of the rOmpBs, WCA, or PBS, followed by a sublethal challenge with R. rickettsii. Five days after challenge, the mice were sacrificed and the rickettsial load in their livers (A), spleens (B), or lungs (C) was determined using R. rickettsii-specific qPCR. The rickettsial load is expressed as the ratio of R. rickettsii ompB to murine actin gene copies (S2 Table), and the results were analyzed by variance (ANOVA) procedure or Kruskal-Wallis test (NPAR1WAY Procedure) according to their normality and homogeneity of variances, followed by between-group comparison with Student-Newman-Keuls Test. Data were presented as mean + SEM. (n = 5). Means with different letters are significantly different (P< 0.05).