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. 2015 Apr 24;10(4):e0124664. doi: 10.1371/journal.pone.0124664

Fig 3. Comparison of immunoprotective efficacy between combination group and individual group.

Fig 3

C3H/HeN mice were immunized with rOmpB-4 combined with C. burnetii CMR (O+CMR-C), C. burnetii CMR alone (CMR-C), or rOmpB-4 alone (rOmpB-4). Day 14 after the last immunization, mice were challenged with R. rickettsii, and mice were sacrificed and their livers (A), spleens (B) and lungs (C) were collected day 5 post challenge. In R. rickettsii-specific qPCR assay, the data was expressed as the ratio of R. rickettsii ompB to murine actin gene copies (S2 Table). The spleen weight of mice in each group was also been determined (D). The significant difference between two groups was compared with the Student’s t-test or Wilcoxon two-sample test according to their normality and homogeneity of variance. All data were presented as mean + SEM. (n = 5). P<0.05 was considered significantly different. *, P<0.05; **, P<0.01; ns, no significance.