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. 2015 Apr 24;10(4):e0124664. doi: 10.1371/journal.pone.0124664

Fig 8. Neutralization of R. rickettsii by sera.

Fig 8

Viable R. rickettsii were incubated with sera from mice immunized with rOmpB-4 combined with C. burnetii CMR (O+CMR-C), rOmpB-4 alone (rOmpB-4), or C. burnetii CMR alone (CMR-C). Sixty minutes later, the mixture of rickettsiae and each serum sample was added into EA.hy 926 cells for a 4-hour incubation. After which the number of R. rickettsii in host cells was determined by R. rickettsii-specific qPCR. Values are presented as the mean with standard deviations. The statistically significant differences among groups were analyzed using the Student’s t-test or Wilcoxon Two-Sample Test based on their normality and equality of variances and are indicated as follows: *, P<0.05; ***, P<0.001; ns, no significance. All data were presented as mean + SD (n = 3).