Fig 4. Curcumin induces p73-dependent apoptosis.

(A) Tu212 (left) and H1299 (right) cells were treated with curcumin and expression of p73 and p73β were measured by Western blotting. (B-C) p73 was inactivated in H1299 (B) and 041 (C) cells by dominant negative p73 and apoptosis was measured by annexin V-PE staining. (D) H1299 cells expressing empty vector or dominant negative p73 were treated with curcumin. Whole cell lysates were immunoblotted with PARP and caspase 3 antibodies. For B and C, average results from three independent experiments were plotted with standard deviation as error bars. p values were determined by student t-test. p<0.05 was considered statistically significant.