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. 2015 Apr 24;10(4):e0124778. doi: 10.1371/journal.pone.0124778

Fig 4. TO901317 (TO) suppressed both ROS production and JNK phosphorylation in the liver of db/db mice.

Fig 4

(A) After 2 weeks treatment of TO or DMSO (control), ROS production in the liver was assessed by confocal microscope with in situ DHE stain. ROS generation was dramatically reduced in the TO-treated db/db mice, compared with that in DMSO controls. But there were no difference between the WT mice. (B) TO treatment (2 weeks) significantly reduced MDA levels in both plasma and liver of db/db mice, but not WT mice. (C) After 2 weeks treatment of TO or DMSO, mice liver tissues were collected for the WB analyses of JNK. JNK phosphorylation was significantly decreased in db/db mice with TO treatment, compared with that in DMSO controls, while the levels of total JNK protein were unaltered. No significant change was observed in either phosphorylation or total JNK protein levels in WT mice. Results in (B) are presented as mean ± SD, *P<0.05. Representatives of 10–15 mice in each group were shown.