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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Vasc Surg. 2014 Oct 24;63(3):795–804. doi: 10.1016/j.jvs.2014.09.036

Figure 5.

Figure 5

Adventitial delivery of Ephrin-B2/Fc stimulates endothelial Eph-B4 phosphorylation. Immunofluorescence of human saphenous vein treated on its adventitial surface with pluronic gel without or with Ephrin-B2/Fc (2 μg/ml) and placed in a bioreactor; analysis of matched samples. (A) Saphenous vein prior to initiation of arterial flow. (B) Saphenous vein treated with pluronic gel alone (24 hr). (C) Saphenous vein treated with Ephrin-B2/Fc (24 hr). Red, phosphotyrosine; Green, Eph-B4; Blue, DAPI. White arrows show co-localization of Eph-B4 and phosphotyrosine in the endothelium. White asterisks, vessel lumen. N=3. Scale bar, 50 μm.