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. Author manuscript; available in PMC: 2015 Oct 23.
Published in final edited form as: Nature. 2015 Feb 2;520(7548):553–557. doi: 10.1038/nature14156

Figure 2. mtDNA stress triggers ISG expression in a cGAS- and STING-dependent fashion.

Figure 2

a-b, ISG expression in Tfam+/− MEFs transfected with the indicated siRNAs (top panels), or WT, cGas−/− (a), and Sting−/− (b) MEFs transfected with TFAM siRNAs (bottom panels). c-d, ISG expression in Tfam+/− MEFs transfected with the indicated siRNAs for 96 hrs. e, Western blots of whole cell and nuclear extracts of WT and Tfam+/− MEFs or Tfam flox ERCre−/+ BMDM exposed to 4OHT for 96 hrs. f-gcGAS−/− MEFs reconstituted with cGAS-HA were transfected with the indicated siRNAs for 96 hrs, then stained with anti-DNA, anti-Hsp60 [Mito], and anti-HA antibodies and imaged. cGAS colocalization scoring was performed as described in the Methods. Error bars indicate ± s.e.m. of triplicates and are representative of three independent experiments. *=p<0.05, **=p<0.01, ***=p<0.001.