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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Am J Hematol. 2015 Feb 5;90(5):386–391. doi: 10.1002/ajh.23952

Figure 3. Increased erythrocyte size resulting from reduction of cyclin A2 levels in erythroid progenitors.

Figure 3

(A, B) Forward scatter (FSC) profiles for a control shRNA (shLuc) or for shRNAs targeting Ccna2 (sh4, sh5) are shown for the nucleated and enucleated fractions at 24 and 48 hours, respectively. Results are representative of at least 3 independent experiments. (C, D) Cell size measurements using phase contrast microscopy images are shown as measured in micrometers (μm). Enucleated and nucleated cells were difficult to separate using phase contrast microscopy and therefore all cells were counted together. At least 100 cells were measured per group and the results are shown at 24 and 48 hours, respectively (ns = non-significant difference, **** = p < 0.0001 using two-sided Student t-test).