Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Mol Psychiatry. 2014 Oct 14;20(9):1069–1078. doi: 10.1038/mp.2014.124

Figure 3. Overexpression of Cyfip1 in vivo results in increased spine density and increased proportion of pyramidal neurons showing a mature spine morphology.

Figure 3

Golgi staining of sections from two month old frontal cortex (layer II-III pyramidal neurons) revealed a (A) qualitative (red arrows; scale bar = 15μm), and (B) quantitative (two-fold increase) in spine density in transgenic mice (Tg#8 and #24 strains) relative to controls. (C) Categorical classification of spines highlight a (D) qualitative (black arrows; scale bar = 3μm), and (E) quantitative shift in the proportion of spines within each category in transgenic mice from either strain relative to controls. In both transgenic strains differences in the overall proportion of mushroom type immature thin spines (Type B; reduced ~50% in both strains), mature stubby spines (Type D; increased 15% in both strains), and abnormal spines (Type E; increased ~ 500% in both strains) were observed relative to control mice. No differences between transgenic mice and wild-type controls were observed for either immature thin spines (Type A) or immature double mushroom-type spines (Type C) ** = p<0.01.