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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Gastroenterology. 2015 Feb 19;148(5):1012–1023.e14. doi: 10.1053/j.gastro.2015.01.045

Figure 4. Shp−/− mice are resistant to alcohol-induced hyperhomocysteinemia.

Figure 4

a, GC/MS analysis of Hcy metabolites. Sera were collected from WT CTRL (black), WT EtOH (blue), Shp−/− CTRL (red), and Shp−/− EtOH (dark green) mice over a 12h/12h light/dark cycle using the NIAAA-binge model. Five individual mice were analyzed in each group. Data are shown in mean ± SEM (n=5). *P < 0.01, Shp−/− vs WT; #P < 0.01, EtOH vs CTRL. ZT, Zeitgeber time.

b, Serum ALT and AST levels in WT CTRL (black), WT EtOH (blue), Shp−/− CTRL (red), and Shp−/− EtOH (dark green) mice over a 12h/12h light/dark cycle using the NIAAA-binge model. *P < 0.01, Shp−/− vs WT; #P < 0.01, EtOH vs CTRL. ZT, Zeitgeber time.

c, qPCR of hepatic Bhmt and Cth mRNA in WT CTRL (black), WT EtOH (blue), Shp−/− CTRL (red), and Shp−/− EtOH (dark green) mice over a 12h/12h light/dark cycle using the NIAAA-binge model. *P < 0.01, Shp−/− vs WT; #P < 0.01, EtOH vs CTRL. ZT, Zeitgeber time.

d, Western blot of hepatic Bhmt, Cth, and FoxA1 protein expression in WT CTRL, WT EtOH, Shp−/− CTRL, and Shp−/− EtOH mice over a 12h/12h light/dark cycle using the NIAAA-binge model. β-Tublin (βTub), loading control.

e, Western blot of hepatic Bhmt, Cth, and FoxA1 protein expression at ZT0 in WT (W), Shp−/− (S), Bhmt−/− (B), and Bhmt−/−Shp−/− (D) mice using the NIAAA-binge model. β-Tublin (βTub), loading control.

f, GC/MS analysis of serum homocysteine levels at ZT0 in WT (W), Shp−/− (S), Bhmt−/− (B), and Bhmt−/−Shp−/− (D) mice using the NIAAA-binge model. Data are shown in mean ± SEM (n =5). Different characters indicate significant differences (P < 0.05).