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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Matrix Biol. 2015 Jan 5;42:56–73. doi: 10.1016/j.matbio.2014.12.002

Fig. 6.

Fig. 6

MMP changes in skin of mutant animals. (a) Transcript levels of Mmp2 and Mmp9 were not affected by loss of Antxr1 (n ≥ 4). (b) Increased protein levels of MMP9 and MMP2 in skin extracts of Antxr1−/− mice. (c) Reduced activity of MMP2 in Antxr1−/− mice (top) by gelatin zymography, and increased mature/cleaved form of MMP14 in mutant skin by western blotting (bottom). (d) Reduced MMP2 activity in skin extracts from Timp2−/− and Antxr1fl/fl::Cdh5Cre (flfl::Cre) mice when compared with WT (+/+), Antxr1fl/fl (fl/fl) or Cdh5Cre (Cre) control mice, but not to the extent seen in Antxr1−/− (−/−) mice (n ≥ 3; *P < 0.05, **P < 0.005). Representative zymography raw data on which this figure is based are shown in Supplemental Fig. 5a. (e) Reduced transcript levels of Timp2 and Timp3 in skin extracts from Antxr1−/− animals (n = 4; *P < 0.05).