Figure 7. Loss of Pak2 leads to deficient lymphopoiesis.

A) Absolute number of CD45.2⁺ common lymphoid progenitors (CLPs: Lin⁻IL7Ra⁺c-Kit^lowSca1^low), (B) number of CD45.2⁺CD3⁺ T cells in thymus, (C) percentage of CD4⁺CD8⁺, CD4⁺CD8⁻, CD4⁻CD8⁺ and CD4⁻CD8⁻ cells within the CD45.2⁺CD3⁺ thymic T cell population, (D) number of CD45.2⁺CD3⁺ T cells in spleen, (E) numbers of CD45.2⁺ BM mature B cells (B220⁺lgM⁺lgD⁺), (F) numbers of CD45.2⁺ BM immature B cells (B220⁺lgM⁺lgD⁻), (G) numbers of CD45.2⁺ BM Pre B (B220⁺lgM⁻lgD⁻CD43⁻CD24^high), Pro B (B220⁺lgM⁻lgD⁻CD43⁺CD24^med) and Pre-Pro B cells (B220⁺lgM⁻lgD⁻CD43⁺CD24⁻), (H) number of peripheral blood CD45.2⁺CD3⁺ T cells and (I) number of peripheral blood CD45.2⁺B220⁺ B cells was determined by flow cytometry. Absolute number of each cell population was obtained by multiplying the percentage with cellularity of thymus or spleen or WBC count. N=4-6 mice/group. Student t test, * indicates p<0.05 between Pak2-KO and WT. Representative data from 2-3 experiments.