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. Author manuscript; available in PMC: 2015 Jun 10.
Published in final edited form as: J Biotechnol. 2014 Aug 14;191:246–249. doi: 10.1016/j.jbiotec.2014.08.008

Figure 1. mPAC activity in D.discoideum acarg- cell lysates.

Figure 1

Lysates of vegetative acarg- and acarg-/A15::mPAC-YFP cells were incubated with ATP/Mg2+ and cAMP phosphodiesterase inhibitors for 20 minutes at 22°C in either darkness or under blue light irradiation for acarg-/A15::mPAC_YFP and only blue light for acarg-. Accumulated cAMP levels were measured at the indicated time points by isotope dilution assay. Means and SD of two experiments performed in triplicate are presented. An asterix denotes a significant difference between cAMP levels in light and dark samples at P<0.002, as determined by a rank sum test (Mann and Whitney, 1947).